Spontaneous Epithelial-Mesenchymal Transition and Resistance to HER-2-Targeted Therapies in HER-2-Positive Luminal Breast Cancer
Figure 1
Spontaneous EMT in SKBR-3 colony clusters is characterized by mature β1-integrin isoform and decreased HER-2.
[a] Images and western blotting for HER-2 and β1-integrin confirm a de-novo phenotype occurred in 2/4 colony clusters (abbreviated “C”), isolated from SKBR-3/EV (SK-EV-C3, EV-C4), and 1/4 colony cluster isolated from SKBR-3/β1 (SK-β1-C1) (dashed boxes). No de-novo phenotype was observed in any JIMT-1 colony cluster. [b] Mammosphere formation assessed and imaged following day 10 showed increased mammosphere formation in SK-EV-C3, EV-C4 and SK-β1-C1 compared to SKBR-3/EV and SKBR-3/β1.