New Insight into Benign Tumours of Major Salivary Glands by Proteomic Approach
Figure 3
WB validation of selected differentially expressed proteins.
ANXA1, CAPG, CRYAB, ESD, GAPDH, IGHG1, SBP1, SOD, S100A9 different expression by immunoblot analysis. Conventional SDS gels were run with protein extracts from PA and WT FNA samples from all samples using 12% resolving capacity. Thirty micrograms of total proteins were loaded into each lane for CAPG, CRYAB, ESD, GAPDH, SBP1, SOD, S100A9 WB, while 10 µg were loaded for ANXA1 and IGHG1 WB. Proteins were transferred onto nitrocellulose membranes and incubated with specific antibodies against the target proteins. Four lanes for PA and WT are shown for each antibody and densitometry of the blots is shown on the top. Statistically significant differences were determined by the Student t test and the p values are indicated.