Soluble Variants of Human Recombinant Glutaminyl Cyclase
Figure 2
Purification of wild type hQPCT.
A, Imidazole gradient (green line) (a) 50 mM, (b) 50–500 mM, (c) 500 mM in FPLC Akta (GE Healthcare). Blue line: UV measure (mAU). B, SDS-PAGE of purified protein fractions. Lane 1: insoluble fraction, lane 2: protein marker, lane 3: total fraction, lane 4: flow-through, lane 5: wash unbound, lanes 6–7: fractions 50 mM imidazole, lanes 8–15: fractions 50–500 mM imidazole.