Popeye Domain Containing 1 (Popdc1/Bves) Is a Caveolae-Associated Protein Involved in Ischemia Tolerance
Figure 1
Localization of Popdc1 in WT cardiomyocytes.
(a) Popdc1 (red labeling) is evident at the lateral membrane (arrow), intercalated discs (arrowhead) and transverse striations (double arrow). (b) Upper panel, co-labeling for Popdc1 (red), Cav3 (green) and vinculin (light blue); on the left, merge of Popdc1 with Cav3 (white) and on the right, merge of Popdc1 with vinculin (white). Lower panel, 3D surface analysis based on Z-stack pictures. Popdc1 (red), Cav3 (light blue), vinculin (green), merge of Popdc1 with Cav3 (violet), merge of Popdc1 with vinculin, yellow. Square size 5×5 µm. (c) Popdc1 (red) and Cav3 (green) co-localize (yellow) in the sarcolemma (arrowhead) and T-tubules (arrows) in isolated adult WT cardiomyocytes (Adult CM), at sites of cell-cell contacts in cultured neonatal WT cardiomyocytes (CMC), and in Popdc1/Cav3 co-transfected COS7 cells at sites of cell adhesion (Asterisk) and inside the cells.