ß-Blocker Timolol Prevents Arrhythmogenic Ca2+ Release and Normalizes Ca2+ and Zn2+ Dyshomeostasis in Hyperglycemic Rat Heart
Figure 3
Effect of timolol treatment of diabetic rats on intracellular global Ca2+ changes.
(A) Representative Ca2+ transients in freshly isolated cardiomyocytes loaded with Fura-2 and field-stimulated at 0.2 Hz, (B) changes in peak amplitude of the fluorescence related with global Ca2+ transients (ΔF340/380 = F340/380Peak-F340/380Basal) (left), basal level of intracellular Ca2+ (middle), and caffeine-induced peak Ca2+ transients elicited in the cardiomyocytes (right), and the effect of timolol-treatment of diabetic rats (DM+TIM) on the time course (time to peak fluorescence, TP and half-decay time of fluorescence (left), DT50 shifts between groups were estimated by a trend-fitting to whole Ca2+ transients evoked by field stimulation of intracellular global Ca2+ changes (right) recorded from freshly isolated ventricular cardiomyocytes (C). Bar graphs represents mean ± SEM of 12–17 cells from at least 5 animals for each group protocol. Significant at *p<0.05 vs. CON.