Activation of GSK-3β and Caspase-3 Occurs in Nigral Dopamine Neurons during the Development of Apoptosis Activated by a Striatal Injection of 6-Hydroxydopamine
Figure 2
Representative merged micrographs of mesencephalon immunostained against NeuN and TH.
The numbers at the left margin of the figures are the days after 6-OHDA lesion. The primary antibodies were a mouse monoclonal anti-NeuN and a rabbit polyclonal anti-TH. The secondary antibodies were sheep anti-mouse IgG (H+L) FITC conjugated and goat anti-rabbit IgG (H+L) Texas conjugated. The rectangles on the panoramic view show the regions that were amplified 40x (details). Scale bar = 1 mm (central panels) and 50 µm (details).