Chronic Alcohol-Induced microRNA-155 Contributes to Neuroinflammation in a TLR4-Dependent Manner in Mice
Figure 2
MicroRNA-155 deficiency protects from alcohol-induced TNFα and MCP1 in mouse cerebellum.
WT (n = 6 or 7) or miR-155-KO (n = 5 or 10) mice were fed with control (PF) or EtOH diet for 5 weeks, respectively. Pro-inflammatory cytokines, TNFα (A) and MCP1 (C) mRNAs were assessed by real-time PCR from whole cerebellar RNA extract and corrected with 18S. TNFα (B) and MCP1 (D) proteins of whole cerebellar lysates were measured by specific ELISAs and corrected with total protein. Bars represent mean±SEM (*, #: p value<0.05 relative to appropriate PF or WT controls, respectively, by Kruskal-Wallis non-parametric test).