Analysis of Trafficking, Stability and Function of Human Connexin 26 Gap Junction Channels with Deafness-Causing Mutations in the Fourth Transmembrane Helix
Figure 11
N206S forms stable hemichannels when expressed with WT.
(A–C) As visible by both EM and Blue Native Western analysis, stable hemichannels only resulted at 2:1 ratios of WT:N206S-V5-His6 (C). The EM image clearly showed distinct hemichannel structures and the hexamer band predominates in the Blue Native (BNGel PAGE) Western (C).