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Bilateral Nerve Alterations in a Unilateral Experimental Neurotrophic Keratopathy Model: A Lateral Conjunctival Approach for Trigeminal Axotomy

Figure 1

Anatomy of the nerve of the posterior orbit and surgical procedures.

A. Anatomy of the branches of the trigeminal nerve (yellow arrows). Two fine ciliary nerve from the ciliary ganglion insert into the posterior sclera aside the optic nerve. B. After lateral canthotomy, two tractional sutures were placed to obtain clear surgical field and allow the eye globe to be dislocated easily. C. The lateral fornix and soft tissue preparation were incised, rotating the eye nasally gently by holding the limbal conjunctiva. D. By gently pushing the nasal fornix using blunt curved forceps, eye globe were rotated to control the eye position, elevate the intraorbital pressure to prevent hemorrhage and enable to see the optic nerve and the branches of the trigeminal nerve. The retinal vessel helped to find the optic nerve and, then the branches of the trigeminal nerve.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0070908.g001