Dynamin-2 Regulates Fusion Pore Expansion and Quantal Release through a Mechanism that Involves Actin Dynamics in Neuroendocrine Chromaffin Cells
Figure 1
Dynamin-2 is highly expressed in bovine adrenal chromaffin cells.
Relative expression of dynamin-1 and -2 was evaluated in BCC and PC12 cells using RT-PCR and western-blot. A: RT-PCR results. Note that in BCC, the amount of dynamin-1 mRNA is significantly lower as compared with the dynamin-2 transcript. Actin was used as an amplification control. A control without reverse transcriptase (RT) was performed in parallel to rule out genomic contamination. Signs+or – indicate, respectively, the presence or absence of RT during amplification. B: Western-blot results. Proteins from bovine brain (BB), BCC, PC12 cells and primary adult mouse fibroblasts (AMF) extracts were separated by electrophoresis on a 4–12% polyacrylamide gel gradient and analyzed by western blot. Note that dynamin-1 level was almost undetectable in BCC. β-actin was used as loaded control.