Regulation of A1 by OX40 Contributes to CD8+ T Cell Survival and Anti-Tumor Activity
Figure 5
Active IKKβ regulates A1 expression in OX40 KO CD8 T cells.
Naive CD8 cells from WT or OX40 KO OT-I TCR transgenic mice were stimulated with peptide and APCs. On day 2/3, T cells were transduced with retroviral vectors expressing GFP, or GFP with CA-IKKβ. On day 5 of primary culture, GFP+ CD8 T cells were sorted and restimulated with APCs and peptide. (a) On various days, GFP+ T cells were isolated and analyzed for A1 and β-actin. Protein amounts were determined by densitometry and are shown relative to the expression in wild-type T cells transduced with control vector (taken as 1). (b) Recall survival, based on recovery of GFP+Vβ5+ T cells over time. Cell numbers present on day 0 were assigned a value of 100%, and cell numbers surviving on days 4, 6 and 8 were used to calculate the percentage recovery. Data represent the mean ± s.d. percentage changes from three separate experiments (p>0.05).