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Alterations and Abnormal Mitosis of Wheat Chromosomes Induced by Wheat-Rye Monosomic Addition Lines

Figure 1

FISH was performed using pAs1 (red) and pSc119.2 (green) as probes on mitotic chromosomes of ‘Mianyang11’.

A pAs1 FISH signals on chromosomes of ‘Mianyang11’, the DAPI-stained chromosomes were converted into a black and white image. B pSc119.2 FISH signals on chromosomes of the same cell in A, the DAPI-stained chromosomes were converted into a black and white image. C Merged image of A and B. Arrows indicate 3D chromosomes with pSc119.2 FISH signals. Chromosomes were counterstained with DAPI (blue).

Figure 1

doi: https://doi.org/10.1371/journal.pone.0070483.g001