Estradiol Regulation of Nucleotidases in Female Reproductive Tract Epithelial Cells and Fibroblasts
Figure 2
Effect of estradiol on nucleotidase gene expression in primary uterine endometrial epithelial cells.
Fold change in mRNA expression compared to Control, which was assigned a value of 1 (dashed line), was analyzed by RT-PCR from purified cultures of polarized EM epithelial cells isolated from 4 patients and treated with estradiol (5×10−8 M) for (A) 2 h or (B) 6 h. NT enzymes are abbreviated as shown in legend to Figure 1. UD = undetectable. Mean and SEM are shown. *, P<0.05 represents significant difference between control and estradiol treatment for NT5C1A at 2 h.