A Simple Method to Extract DNA from Hair Shafts Using Enzymatic Laundry Powder
Figure 3
Effects on the efficiency of real-time PCR of sample amount used for extraction and input template volume.
(A) Second round amplification result (CT value of ETH225). Six sample amount groups (5 mg, 2 mg, 1 mg, 0.5 mg, 0.2 mg, 0.1 mg) and six template volume groups (different template volumes in first round, but same template volume (2 µl) in second round) of Diao™ enzymatic laundry powder. (B) First round amplification result (CT value of HAUT27). Six sample amount groups (5 mg, 2 mg, 1 mg, 0.5 mg, 0.2 mg, 0.1 mg) and six template volume groups of Diao™ enzymatic laundry powder. Because of amplification inhibition factors, no CT value of 5 µl, 2 µl, 1 µl template group and some of 0.5 µl and 0.2 µl template groups were obtained (“undetermined”). Negative controls are not shown in this figure; their CT values were undetermined too.