LC-MS/MS Confirms That COX-1 Drives Vascular Prostacyclin Whilst Gene Expression Pattern Reveals Non-Vascular Sites of COX-2 Expression
Figure 5
COX-2-dependent prostanoid production by aorta versus other mouse tissues in Cox1-/- mice.
(a) PGE2 formation, normalized to tissue mass, was measured by immunoassay in supernatants of Ca2+ ionophore A23187 (50µM)-stimulated tissue segments from Cox1-/- mice. Cox1-/- tissues released a variable amount of PGE2 with low levels in the aorta (highlighted in red), and substantially higher levels in the thymus, intestines, renal medulla, brain and vas deferens. This distribution correlates well with luciferase expression in organs of the Cox2fLuc/+ mouse, as described in Figures 3 and 4. n=6.