Overexpressed DNA Polymerase Iota Regulated by JNK/c-Jun Contributes to Hypermutagenesis in Bladder Cancer
Figure 1
c-Jun activates and binds to the human POLI promoter.
(A) Luciferase activity of the deletions of 5′ flanking region of POLI gene was normalized by β-gal activity. Each bar represents the mean±SD for at least three independent experiments. (B) Schematic representations of the distal POLI promoter region. (C) Transcriptional activities of the deletion or mutant of POLI promoter. *Statistically significant difference compared to the pGL3-275/+63 and pGL3–275/+63MU construct (p<0.01; Student's t-test). (D) EMSA Assay. (E) ChIP assay. Mouse IgG as a negative control. The Input DNA or no DNA added was each used as positive and blank control, respectively.