Sustained-Release Delivery of Prostacyclin Analogue Enhances Bone Marrow-Cell Recruitment and Yields Functional Benefits for Acute Myocardial Infarction in Mice
Figure 1
ONO-1301 enhanced SDF-1 secretion and BMC migration via SDF-1/CXCR4 signaling in vitro.
NHDFs were stimulated with ONO-1301 for 72 hours, then the SDF-1 concentration in the culture medium was determined by ELISA (n = 3 each, *P<0.05 vs. 0 nM). A) Number of BMCs that migrated toward the conditioned medium from ONO-1301-stimulated-NHDFs (0, 10, 100, or 1000 nM ONO-1301, n = 6; 1000 nM+nAB or 1000 nM+AMD, n = 3). *P<0.05 vs. 0 nM, †P<0.05 vs. 10 nM, ‡P<0.05 vs. 1000 nM, §P<0.05 vs. SDF-1. nAB, CXCR4-neutralizing antibody; AMD, CXCR4 antagonist AMD3100. B) Representative pictures of BMCs that had migrated to the medium from ONO-1301-stimulated BMCs. Green, BMCs.