A Serpin Released by an Entomopathogen Impairs Clot Formation in Insect Defense System
Figure 4
Sc-SRP-6 inhibits chymotrypsin-like enzymes by a classical mechanism involving RCL.
A–B: Lineweaver-Burk double reciprocal plots showing that Sc-SRP-6 is a competitive inhibitor of trypsin and α-chymotrypsin. C – D: Formation of SDS-stable complexes of Sc-SRP-6/trypsin and Sc-SRP-6/α-chymotrypsin. An increased SI for Sc-SRP-6 was used (5, 10 and 15 in wells 1, 2 and 3). A serpin/protease complex (asterisk), a serpin (black arrow), a hydrolysed serpin (white arrows) and a degraded serpin (grey arrow) are indicated at specific sites in each gel.