Nuclear Translocation of B-Cell-Specific Transcription Factor, BACH2, Modulates ROS Mediated Cytotoxic Responses in Mantle Cell Lymphoma
Figure 4
Bortezomib treatment increases nuclear localization of BACH2 in bortezomib susceptible cells.
Using ‘AMIRA’ software, confocal images of Jeko (A) and SP53 (B) cells were analyzed to quantitate the BACH2 fluorescence in the cytoplasm and nucleus of control and treated cells. Three random images were analyzed for each cell line. Cytoplasmic and nuclear gating of fluorescence was done using de-segmentation analysis of software using confocal images of both the channels (BACH2, red and Draq5, blue). Representative gated images for cytoplasmic and nuclear marking of control and bortezomib samples for each cell line were shown. Data were collected from analysis of three images and expressed as mean ± SD.