Adenosine 2A Receptor Antagonist Prevented and Reversed Liver Fibrosis in a Mouse Model of Ethanol-Exacerbated Liver Fibrosis
Figure 1
Effect of moderate ethanol intake on CYP2E1 and CCl4 hepatotoxicity.
C57BL/6J mice were allowed free access to 2% (vol/vol) (11% calories) ethanol diet or pair-fed control diet and then exposed to intraperitoneal CCl4 injections over 72 hours, 2 weeks or 5 weeks. (A) Plasma ALT was measured in the acute time course to assess hepatic injury. (B) Plasma AST and ALT were measured at different time points. (C) CYP2E1 protein level in liver was measured by Western blot using whole liver extracts. HSC 70 was used as loading control. Insets show representative image of CYP2E1 Western blots. (D) Activity of CYP2E1 in liver was measured by the hydroxylation of p-nitrophenol in whole liver extracts. Values represent means ± SEM. n = 4 in pair-fed diet, n = 6 in ethanol-fed diet. Values with different alphabetical superscripts were significantly different from each other, p<0.05.