Celastrol Inhibits Lipopolysaccharide-Stimulated Rheumatoid Fibroblast-Like Synoviocyte Invasion through Suppression of TLR4/NF-κB-Mediated Matrix Metalloproteinase-9 Expression
Figure 1
Effect of celastrol on LPS-induced RA-FLS migration and invasion.
A: Chemical structure of celastrol. B: FLSs were incubated with the indicated concentrations of celastrol in serum containing medium for 24 h, and cell viability was measured by MTT assay. *P<0.05, **P<0.01 versus normal control group. C: FLSs were incubated with the indicated concentrations of celastrol for 24 h. Cells were harvested and the cell cycle distribution in the sub-G1 phase was determined by FCM analysis. **P<0.01 versus normal control group. D and E: The migration and invasion abilities of FLSs were detected by cell migration and invasion assay. FLSs were pretreated with the indicated concentrations of celastrol for 24 h. Then, FLSs were allowed to migrate with or without LPS (1 µg/mL) for 24 h. The number of migrating and invasive cells in each chamber was plotted as the mean ± SD in three independent experiments. The results were analyzed by ANOVA. **P<0.01 versus normal control group, ⧫P<0.05, ⧫⧫P<0.01 versus LPS alone-treated group.