Optimization of a Widefield Structured Illumination Microscope for Non-Destructive Assessment and Quantification of Nuclear Features in Tumor Margins of a Primary Mouse Model of Sarcoma
Figure 7
Images of a 50 µm tissue slice demonstrating the correlation between images acquired by the SIM system and H&E histological micrograph.
SIM images were taking using the 4×objective and a frequency of 31.7 mm−1. H&E images were taken using a 2.5×objective and the images were cropped to match one another. Site imaged on tissue contains both cross sectional skeletal muscle (M) and sarcoma tumor (T). The arrows on each image point out a site where tumor tissue was invading into the normal skeletal muscle. Scale bars are 100 µm.