WT1 Promotes Cell Proliferation in Non-Small Cell Lung Cancer Cell Lines through Up-Regulating Cyclin D1 and p-pRb In Vitro and In Vivo
Figure 2
WT1 promotes NSCLC cell proliferation in vitro.
A WT1 expression of NSCLC wild-type cells and NSCLC cells transfected by lentivirus containing pLL3.7 (GFP1), pLV-GFP (GFP2), pLL3.7-WT1-shRNA (WT1-shRNA1, WT1-shRNA2, WT1-shRNA3) and pLV-GFP-WT1 (WT1) by western-blot. B, The viability of NSCLC cells was assessed by CCK-8 assay: overexpression of WT1 promotes the cell viability while inhibition of WT1 expression reduces the effect. Data are represented as mean±SD. *P<0.05, **P<0.001.