CK1δ Kinase Activity Is Modulated by Chk1-Mediated Phosphorylation
Figure 6
CK1δ phosphorylation state influences the effects of CK1δ- and ε-specific inhibitors.
(A) Kinase assays were performed in the presence or absence of either 5 nM of compound 17 [82] or 20 nM of compound 8 [58] using GST-p531–64 (FP267) as substrate and GST-wt CK1δ or GST-CK1δS328A, S370A, T397A as enzymes. * Observed effects are significant at p<0.05. (B) Kinase assays were performed in the presence or absence of either D4476 (300 nM), compound 17 (10 nM) or compound 8 (20 nM) using GST-p531–64 (FP267) as substrate and GST-wt CK1δ alone or in combination with Chk1 as enzymes. * Observed effects are significant at p<0.05.