Differential Regulation of Human 3β-Hydroxysteroid Dehydrogenase Type 2 for Steroid Hormone Biosynthesis by Starvation and Cyclic Amp Stimulation: Studies in the Human Adrenal NCI-H295R Cell Model
Figure 3
Identification of essential transcription factors for human HSD3B2 expression.
Both steroidogenic human placental JEG3 and non-steroidgenic human embryonic kidney HEK293 cells (which do not express HSD3B2 normally) were transfected with the human −1050 HSD3B2 promoter construct and stimulated with different transcription factors. Promoter activation was assessed by the dual luciferase assay (Promega) using pRL-TK as internal control. (A) In JEG3 cells only transcription factors GATA, SF1 and Nur77 together are able to activate the HSD3B2 promoter. (B) Similar results are observed in non-steroidgenic HEK293 cells. Data are expressed in Relative Light Units (RLU). Error bars show the mean±SD of three independent experiments. * p<0.05, ** p<0.01.