Involvement of the Neutral Amino Acid Transporter SLC6A15 and Leucine in Obesity-Related Phenotypes
Figure 7
Effect of high fat diet and leucine supplementation on serum parameters in SLC6A15 males.
A. High fat diet increased plasma insulin levels and this was further exacerbated by supplementation with leucine (ANOVA effect of treatment p<0.001; Scheffe's posthoc test baseline – high fat p<0.001; high fat – high fat with leucine p = 0.002). B. High fat diet did not have a significant effect on blood glucose (ANOVA effect of treatment p = 0.054). C. Thus high fat diet lead to elevated HOMA-IR and this was further exacerbated by leucine supplementation (ANOVA effect of treatment p<0.001; Scheffe's posthoc test baseline – high fat p = 0.010; high fat – high fat with leucine p = 0.007). D. Compared to baseline levels, HF diet lead to increased FFA in both genotypes and this increase was completely reversed by leucine (ANOVA effect of treatment p<0.001; Scheffe's posthoc test baseline – high fat p = 0.029; high fat – high fat with leucine p = 0.045). Data are expressed as means ± SEM; n = 20/group at baseline and 8–11/group after high fat ± leucine.