Epo Receptors Are Not Detectable in Primary Human Tumor Tissue Samples
Figure 2
Specificity to EpoR of anti-EpoR antibodies A82 and M-20.
EpoR protein expression analysis was performed by immunoblotting (IB) with anti-EpoR antibodies A82 or M-20 (lot E2004). Three identical blots using the same lysates were processed then probed with the indicated antibodies at the same time. The EpoR positive controls were a Cos-cell lysate overexpressing a FLAG-tagged version of Human EpoR [6] and UT-7/Epo cells. Negative controls were 769-P cells and COS cells transfected with an empty vector. The position of the 59 kDa full-length EpoR protein is indicated by the arrow. The positions of molecular weight markers are shown. The lower portion of the blot was also probed with anti-cyclophilin as a loading control.