Functional Comparison of Human Adenomatous Polyposis Coli (APC) and APC-Like in Targeting Beta-Catenin for Degradation
Figure 8
APC homo-complexes are different from APCL homo-complexes and APC-APCL hetero-complexes. SW480 cells were transiently transfected on day 1 with 1 µg of either RFP, the indicated APC and APCL constructs tagged with YFP at their N-terminus (see figure 1) or the APCL construct rAPCL730 tagged with RFP at the N-terminus, either alone or in the indicated combinations. Cells were fixed on day 3 and stained with the Hoechst dye. The green, red and blue colours correspond to the fluorescences of YFP, RFP and the Hoechst dye, respectively. Bar, 10 µm.