Functional Divergence in Shrimp Anti-Lipopolysaccharide Factors (ALFs): From Recognition of Cell Wall Components to Antimicrobial Activity
Figure 6
Differential expression of ALF groups in response to an experimental infection.
The transcript abundance of the four ALF Groups (Litvan ALF-A, -B, -C, and -D) was measured by RT-qPCR on circulating hemocytes of shrimp (three groups of three shrimps per condition) injected with the shrimp pathogen Fusarium solani (black boxes), sterile sea water (SSW, grey boxes) or non-injected (white boxes). Analyses were performed 24 and 48 hours after injection. Relative abundances of ALF transcripts are calculated according the 2−ΔΔCq method [32], with the L. vannamei ribosomal protein L40 (Litvan-rpl40, GenBank: FE077602) used as reference gene. Results are expressed as mean values (central squares) ±SE (boxes) and ±SD (whiskers) of relative expressions. Significant differences between conditions for each ALF groups (Student’s t-test, p<0.05) are indicated by different lowercase letters (a, b, and c). The use of a same letter indicates the absence of significant difference, while the use of different letters indicates significant difference.