Attenuation of Krüppel-Like Factor 4 Facilitates Carcinogenesis by Inducing G1/S Phase Arrest in Clear Cell Renal Cell Carcinoma
Figure 4
Altered KLF4 expression affected the cell cycle.
(A) KLF4 blocked the G1/S phase cell cycle transition of 786-O cells. The percentage of 786-O cells at the G0/G1 phase increased significantly to 73.81% but decreased to 22.56% at the S phase. (B) Quantitative analysis is shown for the cell cycle distribution of LV-EGFP and LV-KLF4 in 786-O cells (*P<0.05 versus the LV-EGFP). (C) KLF4 blocked the G1/S phase cell cycle transition of ACHN cells. The percentage of ACHN cells in G0/G1 phase increased significantly to 73.17% but decreased to 20.21% at the S phase cells. (D) Quantitative analysis is shown for the cell cycle distribution of LV-EGFP and LV-KLF4 in ACHN cells (*P<0.05 versus the LV-EGFP). (E) After KLF4 knockdown, the percentage of HKC cells at the G0/G1 phase significantly decreased from 64.23% to 51.08%, the percentage of cells at S phase increased from 26.58% to 38.90%. (F) Quantitative analysis is shown for the cell cycle distribution of Si-NC and Si-KLF4 in HKC cells (*P<0.05 versus the Si-NC). (G) Real-time PCR results of KLF4, cyclin D1, p21WAF1/CIP1, and p27KIP1 expressions in 786-O and ACHN cells after KLF4 overexpression, the expression level of KLF4, cyclin D1, p21WAF1/CIP1, and p27KIP1 in HKC cells was also investigated after KLF4 knockdown. PPIA was used as a loading control. (H) Western blot analysis results of KLF4, cyclin D1, p21WAF1/CIP1, and p27KIP1 expressions in 786-O and ACHN cells after KLF4 overexpression, western blot analysis was also performed to investigate the expressions of cyclin D1, p21WAF1/CIP1, and p27KIP1 after KLF4 knockdown. β-actin was used as a loading control.