Two ENU-Induced Alleles of Atp2b2 Cause Deafness in Mice
Figure 1
Deaf11 and Deaf13 mice harbor point mutations in Atp2b2.
DNA sequence electropherograms of exon 17 in A) +/+ and B) Deaf11/Deaf11 mice. Results are representative of four +/+, 14 +/Deaf11 and 10 Deaf11/Deaf11 mice. DNA sequence electropherograms of exon 9 in C) +/+ and D) Deaf13/Deaf13 mice. Results are representative of two +/+, four +/Deaf13 and four Deaf13/Deaf13 mice. Mutation sites are marked by asterisks. Predicted effect on amino acid sequence is shown below. E) Amino acid sequence alignments for regions surrounding predicted Deaf11 and F) Deaf 13 amino acid substitutions (yellow). Alignments were performed with Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/) using protein sequences Q9R0K7.2, P11506.2, Q01814.2, NP_080758.1, NP_796210.2, AAI09173.1, NP_001033803.3, NP_001087020.1 and CAA09303.1.