Stage-Regulated GFP Expression in Trypanosoma cruzi: Applications from Host-Parasite Interactions to Drug Screening
Figure 5
RT-qPCR and western-blot analysis of GFP expression.
(A) mRNA levels for the GFP and NEO genes in epimastigotes and metacyclic trypomastigotes, as determined by RT-qPCR and shown as relative mRNA levels, with the metacyclic level fixed at 1. (B) Western blot of GFP in epimastigotes (Epi) and metacyclic trypomastigotes (Meta). The “Loading control” (left) is the Ponceau-stained membrane before incubation with anti-GFP antibody (right). Note the detection of GFP only in epimastigotes.