MtnBD Is a Multifunctional Fusion Enzyme in the Methionine Salvage Pathway of Tetrahymena thermophila
Figure 5
Sequence, structure, and phylogenetic analyses of various MtnDs.
(A) Multiple sequence alignment of the MtnD family. Magenta bars show highly conserved cupin motifs I and II, magenta and green triangles show amino acid residues involved in metal binding and catalysis, respectively. (B) Predicted tertiary structure of T. thermophila MtnD. The structure of MtnD was modeled on that of M. musculus MtnD (PDB ID: 1VR3) using Swiss-model. Orange sphere represents a nickel atom. His307, His309, Glu313 and His352 (shown in magenta) form the metal binding site. Phe303, Arg315, and Phe 354 (shown in green) are essential for dioxygenation. (C) Phylogenetic tree based on primary sequences of the MtnD family. Alignments were created with ClustalW and displayed using Treeview. Scale bar indicates a difference of 0.1 substitutions per site. Names and gene accession numbers are as follows: A. gambiae (XP_315627), A. aeolicus (NP_214354), A. thaliana (NP_567443), A. nidulans (XP_664180), B. subtilis (NP_389245), C. elegans (NP_510072), C. variabilis (EFN58452), C. neoformans (XP_572521), D. rerio (NP_955962), D. discoideum (XP_635174), D. melanogaster (NP_001097577), E. cloacae 13047 (YP_003613573), G. kaustophilus (YP_146809), H. sapiens (NP_060739), H. magnipapillata (XP_002165167), K. oxytoca (AAD11793), M. aeruginosa (A8YMJ4), M. brevicollis MX1 (XP_001750499), M. musculus (NP_598813), N. crassa (XP_956660), O. sativa (AAC05511), P. aeruginosa (NP_250375), S. cerevisiae (NP_013722), S. pombe (NP_596475), S. purpuratus (XP_789320), S. elongatus (YP_171626), V. vinifera (CBI26309), V. carteri (XP_002955803), X. tropicalis (NP_001004933). Proteins described in (A) are underscored.