Regulation of Transcriptional Networks by PKC Isozymes: Identification of c-Rel as a Key Transcription Factor for PKC-Regulated Genes
Figure 5
Validation of PKCδ-regulated genes in the CREB transcriptional network.
CREB was silenced in LNCaP, C4, C4-2 or RWPE1 cells using two different RNAi duplexes (#1 and #2). After 48 h, cells were stimulated for 1 h with either 100 nM PMA or vehicle, and mRNA levels of different PKCδ-regulated genes were determined 4 h later using real-time PCR. Results were expressed as fold-increase relative to non-target control RNAi (vehicle-treated). Data represents the mean ± S.E.M. of 3 independent experiments. C, non-target control RNAi duplex. *, p<0.05 vs. non-target control (PMA-treated).