Gauging NOTCH1 Activation in Cancer Using Immunohistochemistry
Figure 7
Relationship of NICD1 staining, NOTCH1 genotype, and karyotype in CLL.
A) Correlation between the fraction of mutated NOTCH1 exon 34 reads and NICD1 staining. B) NICD1 staining in CLLs with and without mutated NOTCH1 alleles. Filled squares correspond to CLLs with the NOTCH1 codon 2514 del(CT) mutation; black squares are tumors with a “high” fraction (>20%) of mutated reads, while gray squares are tumors with a “low” fraction (<5%) of mutated reads. The open square corresponds to a CLL with a nonsense mutation in codon 2444. C) Relationship of NICD1 staining to CLL karyotype. Open squares correspond to tumors with NOTCH1 mutations.