Exposure of Bifidobacterium longum subsp. infantis to Milk Oligosaccharides Increases Adhesion to Epithelial Cells and Induces a Substantial Transcriptional Response
Figure 3
Dose-response of B. longum subsp. infantis ATCC 15697 to 6′sialyllactose and subsequent adherence to HT-29 cells.
(A) Bacterial exposure to original conditions (1 mg/ml; 3 hours), increased duration (1 mg/ml; 6 hours) or increased concentration (2 mg/ml; 1 hour) in comparison to non-treated control bacteria. (B) Exposure to a gradient of 6′sialyllactose was assayed over 3 hours (gray shading) or control and 1 mg/ml for 1 hour exposure (white shading). Results are expressed as fold-change in percent adhesion in relation to the respective control ± standard deviation. adenotes significance in relation to the control; bdenotes significance in relation to 6′SL (1 mg/ml; 6 hr) treatment; p<0.0001.