Lipoxin A4-Induced Heme Oxygenase-1 Protects Cardiomyocytes against Hypoxia/Reoxygenation Injury via p38 MAPK Activation and Nrf2/ARE Complex
Figure 4
LXA4-stimulated HO-1 expression is dependent on Nrf2 translocation.
In A, cardiomyocytes were transfected with Nrf2-specific or nonspecific siRNA and then exposed to H/R injury with or without pretreatment with LXA4. The effects of Nrf2-siRNA on HO-1 gene and protein expression are shown. The amount of PCR products was normalized with β-actin to determine the relative expression ratio (log2 fold) for each mRNA. Protein expression of HO-1 was shown as HO-1/tubulin ratio for each sample. In B, Nrf2 proteins in cytosolic or nuclear extraction of cardiomyocytes were analyzed by using Western blotting. Cytosolic Nrf2 were shown as Nrf2/β-actin ratio, and nuclear Nrf2 were shown as Nrf2/lamin B1 ratio for each sample. In C, Nrf2 translocation was determined by fluorescence microscope in the cells treated with or without H/R injury and with or without pretreatment with LXA4. Results are representative of three independent experiments. In B, *P<0.05, as compared to the cells without treatment.