TLR7/TLR8 Activation Restores Defective Cytokine Secretion by Myeloid Dendritic Cells but Not by Plasmacytoid Dendritic Cells in HIV-Infected Pregnant Women and Newborns
Figure 2
Enhanced IL-10 secretion by TLR7/8 ligand stimulation in HIV-1-infected mothers and newborns.
(A–G) PBMCs from mothers (M) and cord blood (CB) of HIV-1-infected (n = 15) and uninfected controls (n = 15) were stimulated or not (baseline) for 48 h with agonists for TLR2/Pam3Cks4, TLR4/LPS, TLR5/flagellin, TLR3/poly I:C, TLR7/Imiquimode, TLR7/TLR8/CL097 and TLR9/CpG. IL-10 secretion was determined by cytometric bead array. Bars indicate the mean ± SEM. *p≤0.05, **p≤0.01, *** p≤0.001 when compared with the control group; ++p≤0.01 when compared with baseline levels. (H) IL-10 secretion –induced by CL097 from HIV-infected mothers was assessed according to the mother CD4+ T cells number, <350 cells/µL (open circles) or >350 cells/µL (closed circles). Figure represents the median. *p≤0.05 when compared with the CD4+ T cells >350 cells/µL group.