Dynamics and Molecular Determinants of Cytoplasmic Lipid Droplet Clustering and Dispersion
Figure 3
Plin1-coated CLD cluster close to the MTOC and associate with motor proteins.
(A) Representative immunofluorescence images showing the localization of Plin1-coated CLD (red) and γ-Tubulin (green). Hoechst-stained nuclei are shown in blue. The size bar is 10 µm. (B) Kinesin and dynein co-localization with Plin1. Representative immunofluorescence images depicting the localizations of Plin1 and dynein (Dynein+Plin1) or kinesin-5 family members (Kinesin+Plin1) in Plin1-expressing cells are shown. The panels show merged (red = Plin1; green = dynein or kinesins), and Plin1- and dynein- or kinesin-specific images (monochrome). Arrows point to areas of immunofluorescence overlap seen as a yellow color in the composite. Hoechst-stained nuclei are shown in blue. The size bar is 10 µm. (C) Cross-channel Pearson’s correlation coefficients for the overlap of dynein- or kinesin-immunofluorescence with that of Plin1 in control or isoproterenol-treated (Isopro) cells.