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Knockdown a Water Channel Protein, Aquaporin-4, Induced Glioblastoma Cell Apoptosis

Figure 2

Colony formation of LN229 cells after AQP4 reduction.

A. representative images of colony formation for scr/LN229 and siAQP4/LN229 clone 2 cells (200×). The images were taken at the 10th day of the assay. B. Quantitation of colony number in two groups (scr/LN2229 cells group and siAQP4/LN229 clone 2 cells group). Results were from images (100×) of three independent experiments. C. Quantitation of colony size in two groups (scr/LN2229 cells group and siAQP4/LN229 clone 2 cells group). The data was collected by representative images from three repeated experiments (200×) (two-way ANOVA analysis, **p<0.01).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0066751.g002