Induction and Role of Indoleamine 2,3 Dioxygenase in Mouse Models of Influenza A Virus Infection
Figure 2
IDO ablation enhances influenza-specific CD8 T cell responses to PR8 infection.
B6 (WT) or IDO1-KO mice with B6 backgrounds were infected with PR8 virus (50 PFU, i/n) at sub-lethal doses, and 10 days after infection bronchial alveolar lavage (BAL) cells and T cells were counted. A. Representative FACS gating and analysis strategy to detect NP366-specific CD8 T cells using tetramers (upper panel) and intracellular cytokine (IFNγ, IL-10) expression after peptide specific stimulation for 2 hrs ex vivo (lower panel). B–F. Numbers of total BAL (B) and NP366 (C), PA (D), PB1 (E) CD8 and NP311 (F) CD4 specific T cells. Data were analyzed using Student's unpaired t test; *p<0.05, **p<0.01, ***p<0.001, ns, not significant.