GhCAX3 Gene, a Novel Ca2+/H+ Exchanger from Cotton, Confers Regulation of Cold Response and ABA Induced Signal Transduction
Figure 3
Expression of GhCAX3 and sGhCAX3 could impair ABA sensitivity in tobacco plants.
(A) Expression analysis of GhCAX3 and sGhCAX3 in tobacco by RT-PCR. The EF-1α gene was used as an internal control. (B–C) Comparison of seed germination in WT and transgenic lines under NaCl and ABA treatments. (B) Phenotypic analysis of WT and transgenic seeds germinated on 1/2 MS medium for two weeks under normal condition and in presence of 150 mM NaCl as well as 5 µM ABA. (C) Calculation of germinating seeds with green cotyledons. Percentage of germinating seeds with green cotyledons between WT and transgenic lines grown on 1/2 MS medium containing 5 µM ABA was recorded up to 22 days. (D–E) Statistical (D) and phenotypic (E) analysis of WT and transgenic lines after two weeks treatment by ABA. S-10, L-4 and WT seeds were germinated on 1/2 MS medium for 7 days before transferred onto 1/2 MS medium with different concentrations of ABA as indicated. Photograph was taken after 2 weeks growth. (F) Expression analysis of stress-related genes by RT-PCR. Total RNA was extracted from leaf samples at the indicated time points after ABA treatment. Each column in C and D represents an average of four repeats. ** indicates significant differences relative to the control at P<0.01.