Osmotic Stress Changes the Expression and Subcellular Localization of the Batten Disease Protein CLN3
Figure 5
Increased osmolarity upregulates the mRNA level of myc-CLN3.
BHK clone 19 myc-CLN3-expressing cells were grown under isotonic (300 mOsm) or hyperosmotic conditions. Osmolarity was increased at 100 mOsm intervals to 800 mOsm by the addition of either NaCl/urea or sucrose. After being exposed to 800 mOsm for 24 hours, mRNA levels of myc-CLN3 and polyubiquitin were determined by quantitative real-time RT-PCR, normalized to the level of 18S ribosomal RNA, and expressed as fold increase of the isotonic control. Columns and bars represent mean ± S.E.M. (n = 3–6). Statistical significance was determined by one-way ANOVA with Bonferroni’s post-test.