SNaPaer: A Practical Single Nucleotide Polymorphism Multiplex Assay for Genotyping of Pseudomonas aeruginosa
Figure 2
SNaPaer assay: A) position of each marker on the automated electropherogram (a total of 23 markers were included in SNaPaer assay); and B) example of Pseudomonas aeruginosa profile (peaks: orange – ladder; blue – guanine; black – cytosine; green – adenine; red – thymine); C) final SNaP profile of the isolate presented above obtained according to Table 1 (e.g. Ac7, Ac78, … T349) in order to facilitate comparison with MLST data.
When markers are amplified using reverse primers (e.g. A264) the complementary base should be included in the SNaP profile.