Distinct Roles for CBP and p300 on the RA-Mediated Expression of the Meiosis Commitment Gene Stra8 in Mouse Embryonic Stem Cells
Figure 1
Histone acetylation levels at the Stra8 promoter during the RA-induced differentiation of mouse ESCs.
(A) Diagram of the promoter of the Stra8 gene, measured in bp. (B) ChIP analysis of AcH3 and AcH4 occupancy on the RARE1 and RARE2 regions of the Stra8 promoter in ESCs treated with +LIF, −LIF or −LIF/+RA- for 24 h. Isolated chromatin was immunoprecipitated with a nonspecific rabbit normal IgG as a control. The input represents 10% of the material used for immunoprecipitation. (C) The AcH3 and AcH4 levels at the Stra8 promoter, as determined by ChIP analysis in ESCs treated with +LIF, −LIF or −LIF/+RA- for 24 h. Data are means ± SEM (n = 3). P<0.05(*) vs. −LIF ESCs (One-way ANOVA with Turkey-Kramer post hoc test).