Lipopolysaccharide Preconditioning Protects Hepatocytes from Ischemia/Reperfusion Injury (IRI) through Inhibiting ATF4-CHOP Pathway in Mice
Figure 3
ATF4-CHOP pathway and related apoptotic pathway inhibition by LPS preconditioning after IR.
(A) Western-assisted analysis of ATF4, CHOP, Cleaved Caspase-12, Cleaved Caspase-3 and β-Actin. Representative of three experiments. (B) Relative quantities of protein of ATF4, CHOP, Cleaved Caspase-12, Cleaved Caspase-3 to β-Actin, Mean±SD, **P<0.001 versus sham group; ##P<0.001 versus IR group; #P<0.05 versus IR group. (C) immunohistochmistry analysis of CHOP: (a) sham group; (b) IR group and (c) LPS PC+IR group. Positive cells were quantified in six high-power fields (400×), and expressed as percentages of positive cells among total cells. Mean±SD,**P<0.001 versus sham group; ##P<0.001 versus IR group.