Retinoic Acid-Activated Ndrg1a Represses Wnt/β-catenin Signaling to Allow Xenopus Pancreas, Oesophagus, Stomach, and Duodenum Specification
Figure 4
ndrg1a knockdown in Xenopus embryos specifically disturbed pancreas, oesophagus, stomach, and duodenum formation, which phenocopied BMS435 treatment.
(A) 3.5 picomoles of coMO, ndrg1a MO1 or ndrg1a MO2 was vegetally injected into all four blastomeres at four cell stage of development and collected at stages 36 and 42 for whole mount staining with marker genes indicated on the left side or in the images 1–6. (A1–12 and A16–42) Lateral view, head toward the left. (A13–15) Dorsal view. The dorsal structures, such as the neural tube, notochord, and somites were removed after whole mount in situ hybridization. (B) Morphology of ndrg1a MO1 injected and BMS453 treated embryos collected when control siblings developed to stage 42. The numbers of embryos showing the illustrated phenotypes are given in the corresponding images.