Sonic Hedgehog Signaling Pathway Supports Cancer Cell Growth during Cancer Radiotherapy
Figure 5
Effects of SHH signaling agonists on dying cell induced tumor cell growth.
A. Activation of SHH signaling with SHH signaling agonist SAG enhances Panc1Fluc cell growth on irradiated dying Panc1 cells in a dose-dependent manner. Top: signal intensity analysis from bioluminescence image; Bottom: representative bioluminescence images; scale bar represents 1 cm. *represents P<0.05, **represents P<0.01. B. Activation of SHH signaling with SAG enhances HT29Fluc cell growth on irradiated dying HT29 cells in a dose-dependent manner. Top: signal intensity analysis from bioluminescence image; Bottom: representative bioluminescence images; scale bar represents 1 cm. **represents P<0.01. C. Activation of SHH signaling with SHH signaling agonists, N-terminal fragment of Shh, enhances Panc1Fluc cell growth on irradiated dying Panc1 cells. Top: signal intensity analysis from bioluminescence image; Bottom: representative bioluminescence images; scale bar represents 1 cm. *represents P<0.05. D. Activation of SHH signaling with N-terminal fragment of Shh enhances HT29Fluc cell growth on irradiated dying HT29 cells. Top: signal intensity analysis from bioluminescence image; Bottom: representative bioluminescence images; scale bar represents 1 cm. *represents P<0.05.