Effects of HCV on Basal and Tat-Induced HIV LTR Activation
Figure 1
Basal and Tat-induced HIV LTR activation in Huh7.5 cells (A), HepG2 cells (B), and Huh7/β-gal cells (C).
Huh7.5 and HepG2 cells were transfected with an HIV subtype B LTR (LTR-B) luciferase construct in the presence or absence of a Tat-expressing vector. 100 ng of each DNA was transfected per well of a 96-well plate. A luciferase assay was performed at 48 hours post-transfection to quantify LTR activation and was expressed as relative luciferase activity. Huh7/β-gal cells were transfected with or without a Tat expression vector. At 48 hours post-transfection, blue cells were counted after β-gal staining. White bars denote basal (no Tat) LTR activity, and black bars denote Tat-mediated LTR activation.