An Iron 13S-Lipoxygenase with an α-Linolenic Acid Specific Hydroperoxidase Activity from Fusarium oxysporum
Figure 6
HPLC analysis of the products formed from 18∶2(n-6) (A), 18∶2(n-6)-Me (B) and di18∶2(n-6) PC (C).
Reactions were performed in 50 mM Tris/HCl (pH 7.5) containing 10% glycerol, 0.1% sodium desoxycolate and 300 mM NaCl at room temperature for 60 min. Incubations with 18∶2(n-6) and di18∶2(n-6) PC were methylated or transmethylated, respectively. Before extraction the hydroperoxy fatty acids were reduced to their corresponding hydroxyl derivatives. The analysis was carried out by using SP-HPLC. As insets the CP-HPLC of each product is shown.